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Differential Peptide Labeling (iTRAQ) in LC–MS/MS Based Proteomics in Daphnia Reveal Mechanisms of an Antipredator Response
journal contribution
posted on 2015-02-06, 00:00 authored by Christoph Effertz, Stefan Müller, Eric von ElertDaphnia, an important model organism
for studies on ecology and evolution, has become a textbook example
for inducible defenses against predators. Inducible defenses are widespread
in nature, and the underlying molecular mechanisms for this plasticity
in general and in particular in Daphnia are not fully understood. Here, we provide for the first time a
combination of established life-history changes (LHC), which are induced
by chemical cues of a predator (fish kairomones), in Daphnia with differential peptide labeling (iTRAQ)
in LC–MS/MS based proteomics. The aim of the present study
is the elucidation of proteins involved in specific antipredator responses
in a predator–prey system of ecological relevance by high-throughput
proteomics. To obtain a highly specific antifish response of Daphnia, highly purified fish kairomones were applied
in the presence or absence of light. We were able to identify a set
of functional proteins, which are likely to explain the kairomone-mediated
and light-dependent LHC in Daphnia.