A novel and sensitive magnetic polymeric
nanoparticle (MPNP)–polymerase
chain reaction–colorimetry (magneto–PCR–colorimetry)
technique was developed for detection of Vibrio cholerae (V. cholerae). The technique involved
an amplification of V. cholerae DNA
on the surface of an MPNP and then employed the intrinsic catalytic
activity of the MPNP to detect the target gene by colorimetry. An
amino-modified forward primer was covalently labeled onto the MPNP
surface which would bind to PCR product during PCR cycling. By employing
the catalytic activity of the MPNP, the analysis of PCR product bound
MPNP yielded a sensitivity of 103 CFU/mL of V. cholerae in buffer system within 4 h. The specificity
and efficiency of the technique were investigated by using various
bacterial DNAs in drinking and tap water.