Detection and Isolation of Dendritic Cells Using Lewis X‑Functionalized Magnetic Nanoparticles

dendritic cell (DC)-specific intracellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) is a receptor found on DCs that recognizes antigens bearing mannose-rich or fucosylated glycans, including Lewis X (Le^X). Here, we report the fabrication of magnetic nanoparticles coated with multivalent Le^X glycans using Cu (I)-catalyzed azide–alkyne cycloaddition. The resulting nanoparticles are selective and biocompatible, serving as a highly efficient tool for DC detection and enrichment.