Design of Two-Dimensional Layered Double Hydroxide
Nanosheets Embedded with Fe3O4 for Highly Selective
Enrichment and Isotope Labeling of Phosphopeptides
posted on 2018-11-14, 00:00authored byDandan Jiang, Xiqian Li, Qiong Jia
Herein, exfoliated
Cu/Ga layered double hydroxide (LDH) nanosheets
were constructed, over which Fe3O4 nanoparticles
were uniformly spread to maintain the two-dimensional (2D) morphology.
The prepared Fe3O4-LDH exhibited high adsorption
capacity (71.3 μmol g–1 for p-nitrophenylphosphate) and satisfactory enrichment selectivity (β-casein:BSA:ovalbumin
ratio of 1:5000:5000). A low detection limit (0.01 fmol) was obtained
for β-casein phosphopeptides combined with MALDI-TOF MS determinations,
demonstrating that the method was highly sensitive. The recovery of
phosphopeptides was determined to be as high as 93.7%. In addition,
the composite demonstrated specific capture of phosphopeptides from
practical samples, nonfat milk, human saliva, and serum. Fe3O4-LDH also had good performance for quantifying abnormally
regulated phosphopeptides in leukemia patients sera by using an isotope
labeling method. The material consumedly contributed to MS analysis
in phosphoproteome and gave inspirations of using 2D materials in
the field of biomolecules separation.