Design of Thermoresponsive Polymers Toward Antibody Purification
journal contributionposted on 31.05.2019, 00:00 by Kana Nishimori, Masashi Maruyama, Yuzuru Shimazaki, Makoto Ouchi, Hiroshi Yoshida
We proposed a concept of a poly(N-isopropylacrylamide) (PNIPAM)-based thermoresponsive antibody adsorbing material toward antibody purification; the end-functionalized PNIPAM chain can afford to adsorb antibodies below LCST and desorb upon heating due to the thermal response feature. To approach the concept, we synthesized telechelic PNIPAM derivatives through atom transfer radical polymerization (ATRP) and post reactions; an immunoglobulin G (IgG) binding ligand was incorporated at the α-terminal and some carboxylic acid groups for immobilization were embedded at another terminal. The thermoresponsive behaviors were characterized by a temperature-variable turbidity measurement and surface plasmon resonance (SPR) measurement.
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surface plasmon resonanceconceptresponse featureα- terminalAntibody Purificationimmunoglobulin GSPRtemperature-variable turbidity measurementthermoresponsive behaviorsbinding ligandATRPantibody purificationatom transfertelechelic PNIPAM derivativesLCSTend-functionalized PNIPAM chaincarboxylic acid groupsThermoresponsive Polymersadsorb antibodiespost reactions