bm8b01820_si_002.pdf (191.63 kB)
Design of Synthetic Polymer Nanoparticles Specifically Capturing Indole, a Small Toxic Molecule
journal contribution
posted on 2019-03-08, 00:00 authored by Anna Okishima, Hiroyuki Koide, Yu Hoshino, Hiromichi Egami, Yoshitaka Hamashima, Naoto Oku, Tomohiro AsaiSynthetic
polymers are of interest as stable and cost-effective
biomolecule-affinity reagents, since these polymers interact with
target biomolecules both in vitro and in the bloodstream.
However, little has been reported about orally administered polymers
capable of capturing a target molecule and inhibiting its intestinal
absorption. Here, we describe the design of synthetic polymer nanoparticles
(NPs) specifically capturing indole, a major factor exacerbating chronic
kidney disease, in the intestine. N-isopropylacrylamide-based
NPs were prepared with various hydrophobic monomers. The amounts of
indole captured by NPs depended on the structures and feed ratios
of the hydrophobic monomers and the polymer density but not on the
particle size. The combination of hydrophobic and quadrupole interaction
was effective to enhance the affinity and specificity of NPs for indole.
The optimized NPs specifically inhibited intestinal absorption of
orally administered indole in mice. These results showed the potential
of synthetic polymer NPs for inhibiting the intestinal absorption
of a target molecule.