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Design and Synthesis of Activity Probes for Glycosidases

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journal contribution
posted on 21.09.2002, 00:00 by Charng-Sheng Tsai, Yaw-Kuen Li, Lee-Chiang Lo
A new synthetic route was developed for the preparation of activity probe 1 for β-glucosidase in this study. The key glycosidation step begins with benzyl p-hydroxyphenylacetate. Benzylic functionalization for the construction of the trapping device was achieved at later stages. Probe 1 was shown to be able to label the target enzyme. This cassette-like design offers great flexibility for future alterations. It would allow the synthetic scheme to expand to other glycosidase probes with different linker/reporter combinations.