id1c00175_si_001.pdf (254.42 kB)
Download file

Design and Evaluation of New Quinazolin-4(3H)‑one Derived PqsR Antagonists as Quorum Sensing Quenchers in Pseudomonas aeruginosa

Download (254.42 kB)
journal contribution
posted on 17.08.2021, 12:06 by Fadi Soukarieh, Alaa Mashabi, William Richardson, Eduard Vico Oton, Manuel Romero, Shaun N. Roberston, Scott Grossman, Tomas Sou, Ruiling Liu, Nigel Halliday, Irena Kukavica-Ibrulj, Roger C. Levesque, Christel A. S. Bergstrom, Barrie Kellam, Jonas Emsley, Stephan Heeb, Paul Williams, Michael J. Stocks, Miguel Cámara
P. aeruginosa (PA) continues to pose a threat to global public health due to its high levels of antimicrobial resistance (AMR). The ongoing AMR crisis has led to an alarming shortage of effective treatments for resistant microbes, and hence there is a pressing demand for the development of novel antimicrobial interventions. The potential use of antivirulence therapeutics to tackle bacterial infections has attracted considerable attention over the past decades as they hamper the pathogenicity of target microbes with reduced selective pressure, minimizing the emergence of resistance. One such approach is to interfere with the PA pqs quorum sensing system which upon the interaction of PqsR, a Lys-R type transcriptional regulator, with its cognate signal molecules 4-hydroxy-2-heptylquinoline (HHQ) and 2-heptyl-3-hydroxy-4-quinolone (PQS), governs multiple virulence traits and host–microbe interactions. In this study, we report the hit identification and optimization of PqsR antagonists using virtual screening coupled with whole cell assay validation. The optimized hit compound 61 ((R)-2-(4-(3-(6-chloro-4-oxoquina­zolin-3­(4H)-yl)-2-hydroxy­propoxy)­phenyl)­acetonitrile) was found to inhibit the expression of the PA PpqsA promoter controlled by PqsR with an IC50 of 1 μM. Using isothermal titration calorimetry, a Kd of 10 nM for the PqsR ligand binding domain (PqsRLBD) was determined for 61. Furthermore, the crystal structure of 61 with PqsRLBD was attained with a resolution of 2.65 Å. Compound 61 significantly reduced levels of pyocyanin, PQS, and HHQ in PAO1-L, PA14 lab strains and PAK6085 clinical isolate. Furthermore, this compound potentiated the effect of ciprofloxacin in early stages of biofilm treatment and in Galleria mellonella infected with PA. Altogether, this data shows 61 as a potent PqsR inhibitor with potential for hit to lead optimization toward the identification of a PA QS inhibitor which can be advanced into preclinical development.