posted on 2002-03-14, 00:00authored byMilan N. Stojanovic, Tiffany Elizabeth Mitchell, Darko Stefanovic
We report herein a set of deoxyribozyme-based logic gates capable of generating any Boolean
function. We construct basic NOT and AND gates, followed by the more complex XOR gate. These gates
were constructed through a modular design that combines molecular beacon stem-loops with hammerhead-type deoxyribozymes. Importantly, as the gates have oligonucleotides as both inputs and output, they open
the possibility of communication between various computation elements in solution. The operation of these
gates is conveniently connected to a fluorescent readout.