DNAzyme Nanomachine with Fluorogenic Substrate Delivery
Function: Advancing Sensitivity in Nucleic Acid Detection

Hussein, Zain; Nour, Moustapha A. Y.; Kozlova, Anastasia V.; Kolpashchikov, Dmitry M.; Komissarov, Andrey B.; El-Deeb, Ahmed A.

doi:10.1021/acs.analchem.3c04420.s001

DNAzyme Nanomachine with Fluorogenic Substrate Delivery Function: Advancing Sensitivity in Nucleic Acid Detection

journal contribution

posted on 2023-12-11, 17:08 authored by Zain Hussein, Moustapha A. Y. Nour, Anastasia V. Kozlova, Dmitry M. Kolpashchikov, Andrey B. Komissarov, Ahmed A. El-Deeb

We have developed a hook-equipped DNA nanomachine (HDNM) for the rapid detection of specific nucleic acid sequences without a preamplification step. HDNM efficiently unwinds RNA structures and improves the detection sensitivity. Compared to the hookless system, HDNM offers an 80-fold and 13-fold enhancement in DNA and RNA detection, respectively, reducing incubation time from 3 to 1 h.

History

Usage metrics

Keywords

reducing incubation time nucleic acid detection equipped dna nanomachine dnazyme nanomachine rna detection rapid detection detection sensitivity preamplification step hookless system advancing sensitivity 1 h

Licence

CC BY-NC 4.0

DNAzyme Nanomachine with Fluorogenic Substrate Delivery Function: Advancing Sensitivity in Nucleic Acid Detection

History

Usage metrics

Categories

Keywords

Licence

Exports