DNA-Catalyzed Transfer of a Reporter Group
journal contributionposted on 13.12.2006, 00:00 by Tom N. Grossmann, Oliver Seitz
Herein we describe a novel DNA-catalyzed transfer of a reporter group from a donating to an accepting oligo-peptide nucleic acid (PNA), generating high catalytic turnover numbers in a DNA-sequence specific manner. The demonstrated transfer of the Dabcyl group was designed to switch on emission of a fluorescein dye while switching off emission of a rhodamine dye. This setup allows the highly sensitive and selective detection of DNA-sequences in real time.