American Chemical Society
ma0348425_si_004.pdf (38.62 kB)

DNA Alignment at Cationic Lipid Monolayers at the Air/Water Interface

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journal contribution
posted on 2004-05-18, 00:00 authored by Christian Symietz, Marc Schneider, Gerald Brezesinski, Helmuth Möhwald
The interaction of DNA with other DNA and with charged interfaces is of utmost importance for understanding nonviral transfection and DNA diagnostics with optimized chips. This can be studied in detail using Langmuir monolayers of cationic lipids as soft surface of a DNA containing subphase. The positional order of the lipid and the DNA sublattices can be studied by synchrotron X-ray diffraction under continuous variation of parameters such as molecular density or surface pressure. It is shown that DNA binding condenses the membrane surface, and the resulting structure is determined by the interplay of DNA−lipid and DNA−DNA interactions.