Cysteamine-Based Cell-Permeable Zn2+-Specific Molecular Bioimaging Materials: From Animal to Plant Cells
journal contributionposted on 27.11.2013, 00:00 by Sougata Sinha, Gourab Dey, Sunil Kumar, Jomon Mathew, Trinetra Mukherjee, Subhrakanti Mukherjee, Subrata Ghosh
Structure-interaction/fluorescence relationship studies led to the development of a small chemical library of Zn2+-specific cysteamine-based molecular probes. The probe L5 with higher excitation/emission wavelengths, which absorbs in the visible region and emits in the green, was chosen as a model imaging material for biological studies. After successful imaging of intracellular zinc in four different kinds of cells including living organisms, plant, and animal cells, in vivo imaging potential of L5 was evaluated using plant systems. In vivo imaging of translocation of zinc through the stem of a small herb with a transparent stem, Peperomia pellucida, confirmed the stability of L5 inside biological systems and the suitability of L5 for real-time analysis. Similarly, fluorescence imaging of zinc in gram sprouts revealed the efficacy of the probe in the detection and localization of zinc in cereal crops. This imaging technique will help in knowing the efficiency of various techniques used for zinc enrichment of cereal crops. Computational analyses were carried out to better understand the structure, the formation of probe–Zn2+ complexes, and the emission properties of these complexes.