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Crop Management, Genotypes, and Environmental Factors Affect Soyasaponin B Concentration in Soybean

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journal contribution
posted on 23.07.2014, 00:00 by Philippe Seguin, Pratyusha Chennupati, Gilles Tremblay, Wucheng Liu
Soybean [Glycine max (L.) Merr.] seeds contain soyasaponin B, which has putative health benefits. Studies were conducted in multiple environments in Quebec, Canada to determine the effects of genotypes, environments, and seeding dates on soyasaponin B concentration in mature seeds. A growth chamber study was also conducted to determine the impact of high air temperature imposed at specific growth development stages on soyasaponin B in soybeans. Concentrations of individual and total soyasaponin B were determined using high-performance liquid chromatography. Genotype and environment main effects were the main determinants of soyasaponin B concentration in soybean, genotype × environment interactions accounting for less than 5% of the variation for all soyasaponin. Ranking of 20 early maturing soybean genotypes was thus relatively consistent across four environments, total concentration varying between 2.31 and 6.59 μmol g–1. Seeding date consistently impacted soyasaponin B concentrations, early seeding date resulting in the highest concentrations. There was an 11% difference in total soyasaponin B concentration of soybeans seeded in mid-May compared to that in late-June. The response to high air temperature was complex and cultivar specific. High temperature stress restricted to the seed filling stages increased total soyasaponin B concentration in one cultivar by 28% when compared to that in control nonstressed plants; however, in another cultivar high temperature applied during all growth stages reduced total concentration by 27%. Results from the present study thus demonstrate that environmental factors and crop management both impact soyasaponin B concentration in soybeans.