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Download fileCounterflow Gradient Focusing in Free-Flow Electrophoresis for Protein Fractionation
journal contribution
posted on 08.05.2020, 15:07 authored by Matthew Courtney, Ethan Thompson, Tomasz Glawdel, Carolyn L. RenWith
its ability to continuously separate and collect charged analytes,
free-flow electrophoresis (FFE) has become a useful tool for the purification
and real-time analysis of biological mixtures. This work presents
a new free-flow counterflow gradient focusing (FF-CGF) mechanism that
uses a novel velocity gradient to counterbalance electrophoretic migration.
This counterflow gradient is created by simply introducing fluid flow
through the sidewalls of the FFE chamber. The theoretical foundation
and device design for FF-CGF are provided in this work, followed by
implementation and validation, with a detailed discussion on future
opportunities and challenges. Initial results show promise, with the
potential to improve FFE resolution and offer versatility. Compared
to existing focusing techniques, such as free-flow isotachophoresis
and isoelectric focusing, no complex buffer compositions are required.
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Counterflow Gradientfuture opportunitiesFF-CGFfluid flowfree-flow isotachophoresisInitial results show promisebuffer compositionsdevice designfree-flow counterflow gradientFFE resolutionFFE chambercounterflow gradientFree-Flow ElectrophoresisProtein Fractionationoffer versatilityfree-flow electrophoresiselectrophoretic migrationnovel velocity gradient