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Copper(II)-Quenched Oligonucleotide Probes for Fluorescent DNA Sensing

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journal contribution
posted on 27.10.2004, 00:00 by Jens Brunner, Roland Kraemer
A copper(II)-quenched molecular beacon was prepared by attaching fluorescein to the 3‘-end and a copper(II) complex to the 5‘-end of DNA. In the presence of complementary DNA, copper(II) and dye are spatially separated in the duplex and fluorescence increases up to 15-fold, with excellent discrimination of single base mismatches.