posted on 2016-09-30, 00:00authored byHana Vaisocherová-Lísalová, František Surman, Ivana Víšová, Milan Vala, Tomáš Špringer, Maria Laura Ermini, Hana Šípová, Petr Šedivák, Milan Houska, Tomáš Riedel, Ognen Pop-Georgievski, Eduard Brynda, Jiří Homola
Functional
polymer coatings that combine the ability to resist
nonspecific fouling from complex media with high biorecognition element
(BRE) immobilization capacity represent an emerging class of new functional
materials for a number of bioanalytical and biosensor technologies
for medical diagnostics, security, and food safety. Here, we report
on a random copolymer brush surface - poly(CBMAA-ran-HPMAA) - providing high BRE immobilization capacity while simultaneously
exhibiting ultralow-fouling behavior in complex food media. We demonstrate
that both the functionalization and fouling resistance capabilities
of such copolymer brushes can be tuned by changing the surface contents
of the two monomer units: nonionic N-(2-hydroxypropyl)
methacrylamide (HPMAA) and carboxy-functional zwitterionic carboxybetaine
methacrylamide (CBMAA). It is demonstrated that the resistance to
fouling decreases with the surface content of CBMAA; poly(CBMAA-ran-HPMAA) brushes with CBMAA molar content up to 15 mol
% maintain excellent resistance to fouling from a variety of homogenized
foods (hamburger, cucumber, milk, and lettuce) even after covalent
attachment of BREs to carboxy groups of CBMAA. The poly(CBMAA 15 mol
%-ran-HPMAA) brushes functionalized with antibodies
are demonstrated to exhibit fouling resistance from food samples by
up to 3 orders of magnitude better when compared with the widely used
low-fouling carboxy-functional oligo(ethylene glycol) (OEG)-based
alkanethiolate self-assembled monolayers (AT SAMs) and, furthermore,
by up to 2 orders of magnitude better when compared with the most
successful ultralow-fouling biorecognition coatings - poly(carboxybetaine
acrylamide), poly(CBAA). When model SPR detections of food-borne bacterial
pathogens in homogenized foods are used, it is also demonstrated that
the antibody-functionalized poly(CBMAA 15 mol %-ran-HPMAA) brush exhibits superior biorecognition properties over the
poly(CBAA).