posted on 2019-12-11, 20:43authored byHalimeh Aghaei, Ali Reza Solaimany Nazar
In the present study, a double flow-focusing platform
is applied
to evaluate the application of microfluidic devices for the synthesis
of liposomes with a uniform size distribution. The size, polydispersity
index (PDI), and size distribution of liposomes are measured by varying
different factors such as flow rate ratio (FRR), total flow rate (TFR),
total lipid concentration, lipid/cholesterol molar ratio, and various
lipid formulations. A comparison is made between the performance of
double flow-focusing and single flow-focusing devices. The liposomes
in the size range of 50–200 nm are produced with low PDIs of
0.1–0.37. The results show that the smallest particles are
synthesized at the highest FRR and TFR, the lowest total concentration,
a lipid/cholesterol molar ratio of 60:40, and single-lipid formulation.
Also, the double flow-focusing device has the ability to apply higher
TFR values than the single flow-focusing device, which leads to a
higher productivity rate and smaller liposomes.