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Download fileConstruction and Modeling of Concatemeric DNA Multilayers on a Planar Surface as Monitored by QCM‑D and SPR
journal contribution
posted on 2014-07-22, 00:00 authored by Lu Sun, Sofia Svedhem, Björn ÅkermanThe sequential hybridization of a
534 base pair DNA concatemer
layer was monitored by QCM-D and SPR, and the QCM-D data were analyzed
by Voigt viscoelastic models. The results show that Voigt-based modeling
gives a good description of the experimental data but only if shear
viscosity and elasticity are allowed to depend on the shear frequency.
The derived layer thickness, shear viscosity and elasticity of the
growing film give a representation of the DNA film in agreement with
known bulk properties of DNA, and reveal a maximum in film viscosity
when the molecules in the layer contain 75 base pairs. The experimental
data during construction of a 3084 bp DNA concatemer layer were compared
to predictions of the QCM-D response of a 1 μm thick film of
rod-like polymers. A predicted nonmonotonous variation of dissipation
with frequency (added mass) is in qualitative agreement with the experiments,
but with a quantitative disagreement which likely reflects that the
flexibility of such long DNA molecules is not included in the model.