Confocal Microscopy Imaging of NR2B-Containing NMDA Receptors Based on Fluorescent Ifenprodil-Like Conjugates
journal contributionposted on 18.01.2012, 00:00 by Patrice Marchand, Javier Becerril-Ortega, Laetitia Mony, Cédric Bouteiller, Pierre Paoletti, Olivier Nicole, Louisa Barré, Alain Buisson, Cécile Perrio
We describe the synthesis and pharmacological characterization of a first generation of ifenprodil conjugates 4–7 as fluorescent probes for the confocal microscopy imaging of the NR2B-containing NMDA receptor. The fluorescein conjugate 6 displayed a moderate affinity for NMDAR but a high selectivity for the NR2B subunit and its NTD. Fluorescence imaging of DS-red labeled cortical neurons showed an exact colocalization of the probe 6 with small protrusions along the dendrites related to a specific binding on NR2B-containing NMDARs.