Compartment-Specific Labeling of Bacterial Periplasmic Proteins by Peroxidase-Mediated Biotinylation
journal contributionposted on 2018-04-30, 00:00 authored by Uday S. Ganapathy, Lu Bai, Linpeng Wei, Kathryn A. Eckartt, Clarissa M. Lett, Mary L. Previti, Isaac S. Carrico, Jessica C. Seeliger
The study of the bacterial periplasm requires techniques with sufficient spatial resolution and sensitivity to resolve the components and processes within this subcellular compartment. Peroxidase-mediated biotinylation has enabled targeted labeling of proteins within subcellular compartments of mammalian cells. We investigated whether this methodology could be applied to the bacterial periplasm. In this study, we demonstrated that peroxidase-mediated biotinylation can be performed in mycobacteria and Escherichia coli. To eliminate detection artifacts from natively biotinylated mycobacterial proteins, we validated two alternative labeling substrates, tyramide azide and tyramide alkyne, which enable biotin-independent detection of labeled proteins. We also targeted peroxidase expression to the periplasm, resulting in compartment-specific labeling of periplasmic versus cytoplasmic proteins in mycobacteria. Finally, we showed that this method can be used to validate protein relocalization to the cytoplasm upon removal of a secretion signal. This novel application of peroxidase-mediated protein labeling will advance efforts to characterize the role of the periplasm in bacterial physiology and pathogenesis.
detection artifactsperoxidase-mediated biotinylationperoxidase expressionperoxidase-mediated proteinPeroxidase-Mediated BiotinylationBacterial Periplasmic Proteinstyramide azideprotein relocalizationPeroxidase-mediated biotinylationnatively biotinylated mycobacterial proteinsnovel applicationsecretion signalsubcellular compartmentstyramide alkynecytoplasmic proteinsEscherichia colisubcellular compartmentperiplasmbiotin-independent detection