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Comparison of Rapid Methods for Detection of Giardia spp. and Cryptosporidium spp. (Oo)cysts Using Transportable Instrumentation in a Field Deployment
journal contribution
posted on 2012-08-21, 00:00 authored by Hans-Anton Keserue, Hans Peter Füchslin, Matthias Wittwer, Hung Nguyen-Viet, Thuy Tram Nguyen, Narong Surinkul, Thammarat Koottatep, Nadia Schürch, Thomas EgliReliable, sensitive, quantitative, and mobile rapid screening
methods
for pathogenic organisms are not yet readily available, but would
provide a great benefit to humanitarian intervention units in disaster
situations. We compared three different methods (immunofluorescent
microscopy, IFM; flow cytometry, FCM; polymerase chain reaction, PCR)
for the rapid and quantitative detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in a field
campaign. For this we deployed our mobile instrumentation and sampled
canal water and vegetables during a 2 week field study in Thailand.
For purification and concentrations of (oo)cysts, we used filtration
and immunomagnetic separation. We were able to detect considerably
high oo(cysts) concentrations (ranges: 15–855 and 0–240
oo(cysts)/liter for Giardia and Cryptosporidium, respectively) in 85 to 300 min, with FCM being fastest, followed
by PCR, and IFM being slowest due to the long analysis time per sample.
FCM and IFM performed consistently well, whereas PCR reactions often
failed. The recovery, established by FCM, was around 30% for Giardia and 13% for Cryptosporidium (oo)cysts.
It was possible to track (oo)cysts from the wastewater further downstream
to irrigation waters and confirm contamination of salads and water
vegetables. We believe that rapid detection, in particular FCM-based
methods, can substantially help in disaster management and outbreak
prevention.
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Keywords
polymerase chain reactiondisaster situationsPCR reactionsintervention unitsimmunomagnetic separationIFMflow cytometryoutbreak preventionGiardia sppscreening methodsfield campaigndisaster managementRapid Methodswater vegetablesooTransportable Instrumentation300 minCryptosporidium sppanalysis timeField DeploymentReliableFCM2 week field studyGiardia lambliaimmunofluorescent microscopyCryptosporidium parvumirrigation waterscanal water
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