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Comparative Metabolomics Analyses of Plantaricin Q7 Production by Lactobacillus plantarum Q7
journal contributionposted on 2021-09-03, 16:11 authored by Yushan Bu, Hui Yang, Jianxun Li, Yinxue Liu, Tongjie Liu, Pimin Gong, Lanwei Zhang, Shumei Wang, Huaxi Yi
Plantaricin Q7 is a bacteriocin produced by Lactobacillus plantarum Q7 with food preservation potential. Low yield is one of the bottlenecks of the wide application of plantaricin Q7. Nontargeted metabolomics was performed to reveal the mechanism of plantaricin Q7 biosynthesis. The results showed that the composition and abundance of intracellular metabolites varied significantly at key time points of plantaricin Q7 synthesis. Differential metabolic pathways were purine metabolism; pyrimidine metabolism; alanine, aspartate, and glutamate metabolism; amino acid biosynthesis; aminoacyl-tRNA biosynthesis; and ABC transporters. Differential metabolites were xanthine, deoxyadenosine, uracil, 5-methylcytosine, α-ketoglutarate, γ-aminobutyric acid, glutamate, glutamine, and tryptophan. Based on metabolomics information, the putative metabolic synthesis pathway of plantaricin Q7 was proposed. Glutamine, glutamate, and 5-methylcytosine could be critical metabolites and simulate plantaricin Q7 biosynthesis significantly (P < 0.05). Bacteriocin production was investigated by comparative metabolomics in this report, which could help to achieve higher plantaricin Q7 yield by metabolic regulation.
key time pointsfood preservation potentiallactobacillus plantarum q7plantaricin q7 synthesisplantaricin q7 biosynthesisamino acid biosynthesisplantaricin q7 productiondifferential metabolic pathwayscomparative metabolomics analysesplantaricin q7comparative metabolomicstrna biosynthesismetabolic regulationdifferential metabolitesbacteriocin productionaminobutyric acidnontargeted metabolomicsmetabolomics informationwide applicationresults showedpyrimidine metabolismpurine metabolismp low yieldcritical metabolitescould helpbacteriocin producedabc transporters05 ).