Cobalt Phosphate Nanocrystals: A Catalase-Like Nanozyme and In Situ Enzyme-Encapsulating Carrier for Efficient Chemoenzymatic Synthesis of α‑Keto Acid
journal contributionposted on 12.10.2021, 16:04 by Zhenfu Wang, Yang Liu, Xiaoyan Dong, Yan Sun
Chemoenzymatic catalysis combining the traits of chemical and enzymatic catalysis provides tremendous possibilities for the design of biosynthetic pathways utilizing inorganic catalysts and enzymes. However, the efficiency of chemoenzymatic catalysis is usually governed by the synergy and compatibility of the two catalysts. Here, we report for the first time the catalase-like activity of cobalt phosphate nanocrystals (CoPs). By a one-pot biomimetic mineralization with CoPs and l-amino acid oxidase (LAAO) under a mild condition, we have fabricated a hybrid nanobiocatalyst, LAAO@CoPs, for the chemoenzymatic synthesis of α-keto acid. The as-fabricated nanobiocatalyst with directly contacted catalytic sites of the enzyme and nanozyme maximizes the substrate channeling effects for in situ chemical decomposition of the oxidative intermediate, H2O2, during the enzymatic oxidation of l-tryptophan (l-Trp), thus minimizing the H2O2 accumulation and byproduct generation. Benefiting from the superiority of LAAO@CoPs, complete conversion (100.0%) of l-Trp to indole pyruvic acid is achieved, over two times higher than the yield of the free LAAO system (47.6%). Meanwhile, LAAO@CoPs show high stabilities against heat and proteolytic treatments. This work offers a new design approach for constructing a high-performance nanobiocatalyst for cascade reactions, especially for those systems with toxic or reactive intermediates.
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substrate channeling effectspot biomimetic mineralizationcobalt phosphate nanocrystalsindole pyruvic acidamino acid oxidasetwo times highernew design approachfree laao system2 </ subefficient chemoenzymatic synthesissitu chemical decompositionchemoenzymatic synthesisketo acidtwo catalystschemoenzymatic catalysiswork offersusually governedsitu enzymereactive intermediatesproteolytic treatmentsperformance nanobiocatalystoxidative intermediatenanozyme maximizesmild conditionlike nanozymelike activityhybrid nanobiocatalystfirst timeenzymatic oxidationencapsulating carriercomplete conversioncascade reactionsbyproduct generation6 %).0 %)