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Circular Nonuniform Electric Field Gel Electrophoresis for the Separation and Concentration of Nanoparticles
journal contribution
posted on 2022-06-02, 09:03 authored by Lu Liu, Ruilin Yang, Jiaxuan Cui, Peng Chen, Hyok Chol Ri, Huaze Sun, Xiangfan Piao, Minshu Li, Qiaosheng Pu, Maurizio Quinto, John L. Zhou, Hai-Bo Shang, Donghao LiA circular
nonuniform electric field strategy coupled with gel
electrophoresis was proposed to control the precise separation and
efficient concentration of nano- and microparticles. The circular
nonuniform electric field has the feature of exponential increase
in the electric field intensity along the radius, working with three
functional zones of migration, acceleration, and concentration. The
distribution form of electric field lines is regulated in functional
zones to control the migration behaviors of particles for separation
and concentration by altering the relative position of the ring electrode
(outside) and rodlike electrode (inner). The circular nonuniform electric
field promotes the target-type and high-precision separation of nanoparticles
based on the difference in charge-to-size ratio. The concentration
multiple of nanoparticles is also controlled randomly with the alternation
of radius, taking advantage of vertical extrusion and concentric converging
of the migration path. This work provides a brand new insight into
the simultaneous separation and concentration of particles and is
promising for developing a versatile tool for the separation and preparation
of various samples instead of conventional methods.
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various samples insteadelectric field linesbrand new insightalso controlled randomlythree functional zonesfunctional zoneswork providesvertical extrusionversatile tooltaking advantagesize ratiorodlike electrodering electroderelative positioninner ).gel electrophoresisexponential increasedistribution formconventional methodsconcentric converging