am1c07385_si_001.pdf (1.03 MB)
Download fileChitosan/Cellulose-Based Porous Nanofilm Delivering C‑Phycocyanin: A Novel Platform for the Production of Cost-Effective Cultured Meat
journal contribution
posted on 29.06.2021, 19:36 authored by Sohyeon Park, Sungwon Jung, Jiwoong Heo, Won-Gun Koh, Sangmin Lee, Jinkee HongCultured
meat is artificial meat produced via the mass culture
of cells without slaughtering livestock. In the production process
of cultured meat, the mass proliferation for preparing abundant cells
is a strenuous and time-consuming procedure requiring expensive and
excess serum. Herein, C-phycocyanin (C-PC) extracted from blue algae
was selected as a substitute for animal-derived serum and a polysaccharide
film-based platform was developed to effectively deliver C-PC to myoblast
while reducing the cost of cell medium. The polysaccharide platform
has a sophisticated structure in which an agarose layer is capped
on a porous multilayer film formed by molecular reassembly between
chitosan and carboxymethylcellulose (CMC). The porous multilayer film
provides an inner structure in which C-PC can be incorporated, and
the agarose layer protects and stabilizes the C-PC. The completed
platform was easily applied to a cell culture plate to efficiently
release C-PC, thereby improving myoblast proliferation in a serum-reduced
environment during long-term culture. We developed a cell sheet-based
meat model using this polysaccharide platform to evaluate the improved
cost-efficiency by the platform method in the mass proliferation of
cells. This strategy and innovative technology can simplify the production
system and secure price competitiveness to commercialize cultured
meat.