posted on 2023-11-21, 00:03authored byJumi Kim, Hyunwoo Kim
Chiral discrimination of monosaccharides
holds significant importance,
especially given the growing interest of the pharmaceutical industry
in their utilization. However, the majority of existing methods has
predominantly centered around chromatographic techniques. In this
study, we introduce a 19F NMR-based comprehensive approach
for chiral analysis specifically tailored for 15 pairs of aldoses.
This technique involves employing sugar hydrazones containing fluorine
in combination with chiral octahedral gallium and scandium complexes.
By utilizing highly sensitive 19F NMR spectroscopy, the
fluorine label in the sugar hydrazone enables accurate differentiation
between d and l enantiomers. The efficiency of the
newly developed method was demonstrated through its successful application
in both quantitative and qualitative analyses of mixtures containing
various monosaccharides.