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Characterization of the Binding Interaction between the Oncoprotein Gankyrin and a Grafted S6 ATPase

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journal contribution
posted on 17.12.2015, 05:44 by Alex M. Chapman, Bryce E. Rogers, Brian R. McNaughton
A complex with the C-terminal portion of the proteosomal subunit S6 ATPase is the only available structure of a protein–protein interaction involving the oncoprotein gankyrin. However, difficulties associated with recombinant expression of S6 ATPase alone, or truncations thereof, have limited our understanding of this assembly. We replaced the C-terminal portion of FtsH from Escherichia coli with the structurally homologous C-terminal portion of S6 ATPase and used this grafted protein to characterize the gankyrin–S6 ATPase binding interaction by isothermal titration calorimetry.