American Chemical Society
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Characterization of a Bisphenol A Specific Yeast Bioreporter Utilizing the Bisphenol A‑Targeted Receptor

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journal contribution
posted on 2013-11-05, 00:00 authored by Johanna Rajasärkkä, Marko Virta
The first Saccharomyces cerevisiae yeast bioreporter for analysis of a single endocrine disrupting compound, bisphenol A (BPA), was developed. The bioreporter contains mutated human estrogen receptor α (hERα), called bisphenol A-targeted receptor (BPA-R). The BPA-R bioreporter was characterized with mixtures of estrogenic chemicals and tested with spiked influent wastewater samples. The detection limit for BPA was 4.2-fold lower (0.107 μM, i.e., 24 μg L–1), while that of the native hormone 17β-estradiol (E2) (1 μM, i.e., 272 μg L–1) was 166,000-fold higher compared to the wild type hERα bioreporter. The BPA-R bioreporter responded only to BPA in a chemical cocktail and spiked concentrated wastewater samples with high concentrations of other estrogenic chemicals. As a conclusion, wastewater and other environmental water samples can be concentrated and specifically analyzed for BPA without risk of the mixture effect caused by other estrogenic chemicals. The BPA-R bioreporter is a robust and cost-efficient choice for high-throughput monitoring of BPA and its bioavailability in complex samples.