posted on 2020-03-17, 14:38authored byFei Wang, Wen-He Zhang, Juan Zhao, Wen-Jia Kang, Shengyang Wang, Biao Yu, Hai-Xue Pan, Gong-Li Tang
Miharamycins
are peptidyl nucleoside antibiotics with a unique
branched C9 pyranosyl amino acid core and a rare 2-aminopurine moiety.
Inactivation of 19 genes in the biosynthetic gene cluster and identification
of several unexpected intermediates suggest an alternative biosynthetic
pathway, which is further supported by feeding experiments and in vitro characterization of an unusual adenylation domain
recognizing a complex nucleoside derivative as the substrate. These
results thereby provide an unprecedented biosynthetic route of high-carbon
sugar catalyzed by atypical hybrid nonribosomal peptide synthetase–polyketide
synthase.