Characterization of MYC-Induced Tumorigenesis by in Situ Lipid Profiling
journal contributionposted on 2013-05-07, 00:00 authored by Richard H. Perry, David I. Bellovin, Emelyn H. Shroff, Ali I. Ismail, Tahera Zabuawala, Dean W. Felsher, Richard N. Zare
We apply desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to provide an in situ lipidomic profile of genetically modified tissues from a conditional transgenic mouse model of MYC-induced hepatocellular carcinoma (HCC). This unique, label-free approach of combining DESI-MSI with the ability to turn specific genes on and off has led to the discovery of highly specific lipid molecules associated with MYC-induced tumor onset. We are able to distinguish normal from MYC-induced malignant cells. Our approach provides a strategy to define a precise molecular picture at a resolution of about 200 μm that may be useful in identifying lipid molecules that define how the MYC oncogene initiates and maintains tumorigenesis.