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Download fileCharacterization of Gelatin/Gellan Gum/Glycol Chitosan Ternary Hydrogel for Retinal Pigment Epithelial Tissue Reconstruction Materials
journal contribution
posted on 01.09.2020, 13:36 authored by Min A. Rim, Joo Hee Choi, Ain Park, Jina Youn, Sumi Lee, Na Eun Kim, Jeong Eun Song, Gilson KhangThe
cellular transplantation approach to treat damaged or diseased
retina is limited because of poor survival, distribution, and integration
of cells after implantation to the sub-retinal space. To overcome
this, it is important to develop a cell delivery system. In this study,
a ternary hydrogel of gelatin (Ge)/gellan gum (GG)/glycol chitosan
(CS) is suggested as a cell carrier for retinal tissue engineering
(TE). Physicochemical properties such as porosity, swelling, sol fraction,
and weight loss were measured. The mechanical study was performed
with compressive strength and viscosity to confirm applicability in
retinal TE. An in vitro experiment was carried out
by encapsulating ARPE-19 in the designed hydrogel to measure viability
and expression of retinal pigment epithelium-specific proteins and
genes. The results showed that the ternary hydrogel system improves
the mechanical properties and stability of the composite. Cell growth,
survival, adhesion, and migration were enhanced as the CS was incorporated
into the matrix. In particular, real-time polymerase chain reaction
analysis showed a markedly improved specific gene expression rate
in the Ge/GG/CS. Therefore, it is expected that the ternary system
suggested in this study can be used as a promising material for retinal
TE.
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Keywords
tissue engineeringweight losssol fractionpolymerase chain reaction analysissurvivalGGsub-retinal spaceternary hydrogelgene expression rateGemeasure viabilityternary hydrogel systemcell carrierternary systemCScell delivery systemtransplantation approachencapsulating ARPE -19cell growthRetinal Pigment Epithelial Tissue R...pigment epithelium-specific proteinsTEPhysicochemical properties