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Cellulose Nanofibrils as Assay Substrates for Cellulases and Lytic Polysaccharide Monooxygenases
journal contribution
posted on 2020-07-02, 20:29 authored by Alixander Perzon, Benedikt M. Blossom, Claus Felby, Tina Jeoh, Alex Hitomi, Peter Ulvskov, Bodil JørgensenA facile
method for preparing colloidal cellulose nanofibrils (CNFs)
from potato tubers is presented comprising alkaline treatment to remove
noncellulosic polysaccharides, followed by hypochlorite oxidation
and homogenization. The CNF consisted of ∼94% cellulose, and
carboxyl groups on the surface of CNF prevented their agglomeration.
Aqueous suspensions of CNF had up to two times higher optical transparency
than phosphoric acid swollen cellulose (PASC). The motivation for
preparing CNF was to use it as a substrate for probing cellulose-degrading
enzymes. It was shown that a lytic polysaccharide monooxygenase (LPMO)
is active on CNF under formation of the same products as with other
cellulose substrates. Moreover, CNF responded to cellulase treatment
in a similar way to microcrystalline cellulose (Avicel), while PASC
was rapidly broken down. Thus, a new assay substrate for these enzymes
is presented with a unique combination of naturelike fibrillar structures
and high optical transparency, which opens up for new types of analyses.
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Cellulose Nanofibrilsnoncellulosic polysaccharidescellulose-degrading enzymesphosphoric acidlytic polysaccharide monooxygenaseLytic Polysaccharide Monooxygenasesmicrocrystalline celluloseassay substratehypochlorite oxidationnaturelike fibrillar structurespotato tuberscellulose nanofibrilsPASCcellulose substratesCNFAqueous suspensionsAssay Substratescellulase treatmentcarboxyl groupsLPMO
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