sc9b05400_si_001.pdf (805.46 kB)
Cellulase Immobilization onto Magnetic Halloysite Nanotubes: Enhanced Enzyme Activity and Stability with High Cellulose Saccharification
journal contribution
posted on 2019-12-27, 13:43 authored by Devendra Sillu, Shekhar AgnihotriA quest for efficient biotransformation of cellulosic
material
into sustainable biochemical products for recent biotechnological
interventions is currently under way. Herein, we report the fabrication
of nanobiocatalyst (NBC) employing halloysite nanotubes (HNTs) as
a template for immobilizing cellulase enzyme, which catalyzed the
hydrolysis of cellulose into glucose. Magnetic character was imported
to HNTs by in situ anchoring of iron oxide nanoparticles,
onto which cellulase was immobilized using aminosilane surface-functional
chemistry. Characterization studies revealed nanobiocatalyst to be
extremely stable during heterogeneous catalysis without compromising
their catalytic activity. The optimization of process parameters yielded
∼93.5% activity of cellulase with high enzyme loading (111.6
mg·g–1 HNTs) after immobilization. Immobilized
cellulase displayed superior stability at elevated temperatures (≥60°C)
and storage capability compared with their free forms. The NBC even
retained ∼68.2% of its original activity after seven consecutive
uses with a minimum yield of 25.4 mg glucose·g–1 cellulose and was 100% recoverable using a magnet. Displaying a
high ionic-liquid tolerance ability is concurrent with superior catalytic
potential against CMC and extracted cellulose (bagasse), and achieving
∼50.2% saccharification and 0.56 g glucose·g–1 cellulose within 48 h of continuous operation establishes the commercial
viability of using cellulase-immobilized HNTs for efficient cellulose
hydrolysis. The sustainability and eco-friendly endeavors in this
approach would pave the way toward valorization and consolidated bioprocessing
of cellulose materials.
History
Usage metrics
Categories
Keywords
48 hcellulose materialscellulase-immobilized HNTsstorage capabilityHigh Cellulose Saccharificationcellulose hydrolysisCMCprocess parametersMagnetic Halloysite NanotubesCharacterization studiesEnhanced Enzyme Activityiron oxide nanoparticlesbiotechnological interventionseco-friendly endeavorsNBCglucosehalloysite nanotubesimmobilizing cellulase enzymeMagnetic characterionic-liquid tolerance abilityImmobilized cellulasecellulosic materialCellulase Immobilizationaminosilane surface-functional chemistry
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC