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Cellular Internalization of Dissolved Cobalt Ions from Ingested CoFe2O4 Nanoparticles: In Vivo Experimental Evidence

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journal contribution
posted on 2013-05-21, 00:00 authored by Sara Novak, Damjana Drobne, Miha Golobič, Jernej Zupanc, Tea Romih, Alessandra Gianoncelli, Maya Kiskinova, Burkhard Kaulich, Primož Pelicon, Primož Vavpetič, Luka Jeromel, Nina Ogrinc, Darko Makovec
With a model invertebrate animal, we have assessed the fate of magnetic nanoparticles in biologically relevant media, i.e., digestive juices. The toxic potential and the internalization of such nanoparticles by nontarget cells were also examined. The aim of this study was to provide experimental evidence on the formation of Co2+, Fe2+, and Fe3+ ions from CoFe2O4 nanoparticles in the digestive juices of a model organism. Standard toxicological parameters were assessed. Cell membrane stability was tested with a modified method for measurement of its quality. Proton-induced X-ray emission and low energy synchrotron radiation X-ray fluorescence were used to study internalization and distribution of Co and Fe. Co2+ ions were found to be more toxic than nanoparticles. We confirmed that Co2+ ions accumulate in the hepatopancreas, but Fen+ ions or CoFe2O4 nanoparticles are not retained in vivo. A model biological system with a terrestrial isopod is suited to studies of the potential dissolution of ions and other products from metal-containing nanoparticles in biologically complex media.