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Cathepsin G Activity as a New Marker for Detecting Airway Inflammation by Microscopy and Flow Cytometry
journal contribution
posted on 2019-02-19, 16:18 authored by Matteo Guerra, Dario Frey, Matthias Hagner, Susanne Dittrich, Michelle Paulsen, Marcus A. Mall, Carsten SchultzMuco-obstructive
lung diseases feature extensive bronchiectasis
due to the uncontrolled release of neutrophil serine proteases into
the airways. To assess if cathepsin G (CG) is a novel key player in
chronic lung inflammation, we developed membrane-bound (mSAM) and
soluble (sSAM) FRET reporters. The probes quantitatively revealed
elevated CG activity in samples from 46 patients. For future basic
science and personalized clinical applications, we developed a rapid,
highly informative, and easily translatable small-molecule FRET flow
cytometry assay for monitoring protease activity including cathepsin
G. We demonstrated that mSAM distinguished healthy from patient cells
by FRET-based flow cytometry with excellent correlation to confocal
microscopy data.
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confocal microscopy dataairwaycathepsin Gsampleplayertranslatable small-molecule FRET flow cytometry assayInflammationmembrane-boundNewFlow Cytometry Muco-obstructive lung diseases featurereporterapplicationinflammationAirwayneutrophil serine proteasesbronchiectasiCGcorrelationMicroscopyreleasesSAMCathepsin G Activityprobemonitoring protease activityfuturemSAMMarkerFRET-based flow cytometry
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