Catalytic Activity of Peptide–Nanoparticle Conjugates Regulated by a Conformational Change
journal contributionposted on 19.09.2017, 00:00 by Dorian J. Mikolajczak, Jason L. Heier, Boris Schade, Beate Koksch
Herein, we present the design and synthesis of a catalytically active peptide-nanoparticle conjugate whose activity is regulated by a defined conformational change in the self-assembled peptide monolayer. A catalytically active peptide, designed after the heterodimeric α-helical coiled-coil principle was immobilized onto gold nanoparticles, and kinetic studies were performed according to the Michaelis–Menten model. The formed peptide monolayer at the gold nanoparticle surface accelerated p-nitrophenylacetate (pNPA) hydrolysis by 1 order of magnitude compared to the soluble peptide while exhibiting no defined secondary structure as determined by infrared (IR) and circular dichroism (CD) spectroscopy. Addition of the complementary peptide-induced coiled-coil formation while significantly hindering the pNPA hydrolysis catalyzed by the peptide–nanoparticle conjugate. The heptad repeat sequence of a coiled-coil opens up the opportunity for regulation of conformation and thus catalytic activity of peptide–nanoparticle conjugates upon interaction with a complementary coiled-coil sequence. Strategies of regulation of catalytic activity by interaction with a complementary cofactor/ligand are well-established in nature and are introduced here into rationally designed peptide–nanoparticle conjugates.
Read the peer-reviewed publication
peptide-nanoparticle conjugateCatalytic Activitygold nanoparticle surfacegold nanoparticlesself-assembled peptide monolayerIRpeptide monolayercoiled-coil sequencepeptide-induced coiled-coil formationp NPAConformational Change Hereinheterodimeric α- helical coiled-coil principlep NPA hydrolysis1 order