Cardiac Troponin I: Ultrasensitive Detection Using Faradaic Electrochemical Impedance
journal contributionposted on 2018-12-12, 14:03 authored by Elaine Spain, Serena Carrara, Kellie Adamson, Hui Ma, Richard O’Kennedy, Luisa De Cola, Robert J. Forster
An electrochemical biosensor for the detection of cardiac troponin I, cTnI, an important cardiac biomarker, is described. A combination of a novel monoclonal antibody, mAb20B3, and a novel Ir(III)-based metal complex was used for detection using faradaic electrochemical impedance spectroscopy. A limit of detection of 10 ag/mL was achieved, which is significantly lower than established assays. The ability to detect these ultralow concentrations enables rapid and early stage detection of cardiac events and opens up the possibility of developing a point-of-care device.