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Download fileCarbon Dots Derived from Citric Acid and Glutathione as a Highly Efficient Intracellular Reactive Oxygen Species Scavenger for Alleviating the Lipopolysaccharide-Induced Inflammation in Macrophages
journal contribution
posted on 01.09.2020, 13:33 authored by Huibo Wang, MengLing Zhang, Yurong Ma, Bo Wang, Hui Huang, Yang Liu, Mingwang Shao, Zhenhui KangReactive
oxygen species (ROSs), acting as functionalized molecules in intracellular
enzyme reactions and intercellular communication of immune response,
play vital roles in biological metabolism. However, the inevitably
excessive ROS-induced oxidative stress is harmful for organ tissue,
causing unexpected local anaphylaxis or inflammation. Here, we demonstrate
carbon dots (CDs), made of citric acid and glutathione via one-step
hydrothermal method, as a highly efficient intracellular ROS scavenger
for alleviating the lipopolysaccharide (LPS)-induced inflammation
in macrophage. These CDs have broad-spectrum antioxidant properties
and the total antioxidant activity exceeds 51.6% higher than that
of the precursor, namely, glutathione, in the same mass concentration.
Moreover, their antioxidative performance in macrophage inflammation
induced by LPS was investigated, and it was found that CDs can efficiently
remove up to 98% of intracellular ROS, notably inhibiting nuclear
factor kappa-light-chain-enhancer of activated B cells (NF-κB)
signaling pathway, and decrease the expression level of inflammatory
factor IL-12. Our results suggested that CDs can serve as a highly
efficient intracellular ROS scavenger and could be employed to cope
with oxidative stress-induced diseases.
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Keywords
functionalized moleculesmacrophage inflammationNF -κBB cellsROS-induced oxidative stressLPSintercellular communicationEfficient Intracellular Reactive Ox...glutathionemass concentrationantioxidant activityintracellular ROS scavengerintracellular ROSintracellular enzyme reactionsbroad-spectrum antioxidant propertiesMacrophages Reactive oxygen speciescarbon dotsantioxidative performancefactor kappa-light-chain-enhancerexpression levelhydrothermal methodorgan tissueoxidative stress-induced diseasesLipopolysaccharide-Induced Inflammationcarbon Dots Derivedfactor IL -12.