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Cancer Cell Lysate Entrapment in CaCO3 Engineered with Polymeric TLR-Agonists: Immune-Modulating Microparticles in View of Personalized Antitumor Vaccination

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posted on 20.04.2017, 00:00 by Lien Lybaert, Keun Ah Ryu, Lutz Nuhn, Riet De Rycke, Olivier De Wever, Alfred C. Chon, Aaron P. Esser-Kahn, Bruno G. De Geest
Here, we report on a strategy to encapsulate cancer cell lysate in immunomodulating microparticles in view of their potential use in personalized anticancer vaccination. In a first step, cancer cell lysate is entrapped into porous calcium carbonate (CaCO3) microparticles by a coprecipitation reaction. Second, a polymer substituted with a small molecule TLR7/8-agonist is adorbed onto the surface of the microparticles. Relative to soluble cell lysate, microparticle encapsulation yielded higher uptake of cell lysate by dendritic cells in vitro. Also, microparticle-adsorbed polymeric TLR7/8-agonists retained their TLR-triggering capacity resulting in immunogenic vaccine particles. We anticipate this strategy might hold potential to turn patient-derived tumor tissue, comprising the patient’s own mutanome, into potent personalized anticancer vaccines.

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