co9b00154_si_001.pdf (583 kB)
Calculating Resin Functionalization in Solid-Phase Peptide Synthesis Using a Standardized Method based on Fmoc Determination
journal contribution
posted on 2019-10-28, 17:37 authored by Othman Al Musaimi, Alessandra Basso, Beatriz G. de la Torre, Fernando AlbericioSolid-phase synthesis
is the method of choice for peptide preparation
in both research and industrial settings. The whole synthetic process
is governed by the initial functionalization of the resin. Although
the literature provides several methods to determine such functionalization,
the addition of an Fmoc-amino acid and the posterior spectrophotometric
measurement of the dibenzofulvene adduct formed after Fmoc removal
is the most widely used for this purpose. However, a range of molar
extinction coefficient (ε) values and even wavelengths are currently
used in the field, with no standardization of the method. Here, we
propose a single-point standardization method that involves a standard
solution of the corresponding amino acid to be checked that is prepared
freshly at the time of the analysis.