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Caffeine-Mediated Detachment of Mutagenic Ethidium from Various Nanoscopic Micelles: An Ultrafast Förster Resonance Energy Transfer Study
journal contribution
posted on 2012-07-12, 00:00 authored by Soma Banerjee, Masanori Tachiya, Samir Kumar PalIn the present study we explore the efficacy of caffeine
in dissociating
the ethidium (Et) molecule, a model DNA-intercalator as well as a
potential mutagen, from nanometer sized micelles of various charges.
Steady-state and picosecond-resolved spectroscopic studies on the
detachment of Et from various biomimicking micelles of different charges
(cationic hexadecyltrimethylammonium bromide (CTAB), neutral (polar)
Triton X-100 (TX-100), and anionic sodium dodecyl sulfate (SDS)) reveal
the specificity of the caffeine molecule for carrying out such dissociation.
The picosecond-resolved Förster resonance energy transfer (FRET)
studies between a DNA minor groove binder dye Hoeschst 33258 (H258,
donor) and Et (acceptor) have been employed to investigate the alteration
in their association in the presence of caffeine at the molecular
level. Analysis of our experimental results employing both the generalized
and the extended version of the well-known “Infelta–Tachiya
model” vividly illustrates how the distribution of Et along
with the equilibrium constant of its solubilization in the micelle
changes in the presence of caffeine in aqueous solution. Finally,
our fluorescence micrographs of squamous epithelial cells validate
the alteration of FRET efficiency between the donor and the acceptor
due to the release of the latter in the presence of caffeine.
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epithelial cellssodium dodecyl sulfatechargealterationgroove binder dye Hoeschst 33258CTABVarious Nanoscopic Micellesbiomimicking micellesacceptorcationic hexadecyltrimethylammonium bromidefluorescence micrographspresencemicelle changesFoDNAcaffeine moleculeTXdonorMutagenic EthidiumSDSFRET efficiencymodel
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