Ca²⁺-Responsive Glyco-insulin

Chemical modification of peptides and proteins, such as PEGylation and lipidation, creates conjugates with new properties. However, they are typically not dynamic or stimuli-responsive. Self-assembly controlled by a stimulus will allow adjusting properties directly. Here, we report that conjugates of oligogalacturonic acids (OGAs), isolated from plant-derived pectin, are Ca²⁺-responsive. We report the conjugation of OGA to human insulin (HI) to create new glyco-insulins. In addition, we coupled OGA to model peptides. We studied their self-assembly by dynamic light scattering, small-angle X-ray scattering, and circular dichroism, which showed that the self-assembly to form nanostructures depended on the length of the OGA sequence and Zn²⁺ and Ca²⁺ concentrations. Subcutaneous administration of OGA12–HI with Zn²⁺ showed a stable decrease in blood glucose over a longer period of time compared to HI, despite the lower receptor binding affinity.