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C18:1 Improves the Freeze-Drying Resistance of Lactobacillus plantarum by Maintaining the Cell Membrane
journal contribution
posted on 2020-08-05, 14:40 authored by Guangqiang Wang, Pengyu Chen, Xiaoqing Yu, Yongjun Xia, Li-Tang Yan, Lianzhong AiIncreasing
knowledge about lactic acid bacteria as fermentation
starters and probiotics to improve health has led to a growing awareness
of their application potential. Despite a long history of applying
cryoprotectants, the maintenance of probiotic viability is still a
major challenge. In this study, we implemented a strategy and explored
its mechanisms in detail. We found that the survival rates after freeze-drying
were positively correlated with the relative concentration of the
octadecenoic acid (C18:1) and with the ratio of unsaturated to saturated
FAs (U/S ratio). The addition of C18:1 significantly improved the
survival of L. plantarum after freeze-drying. Contrary
to the most commonly used cryoprotectants, the addition of C18:1 did
not affect the glass transition temperature or collapse temperature.
We predicted that the cell membrane characteristics would be significantly
degraded during the drying stage, but C18:1 can effectively maintain
the cell membrane integrity and fluidity. Our experiments confirmed
those predictions, and simultaneously found that the enzyme activities
of key enzymes of glucose metabolism were increased compared with
the control group. These finding indicate that C18:1 might serve as
a lyoprotectant to maintain the cell membrane integrity and fluidity,
and thereby increasing the survival rate of L. plantarum after freeze-drying. This study constitutes a strategy to safeguard
bacterial viability.
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FAglucose metabolismsurvival ratescell membrane integrityLactobacillus plantarumsurvival ratefluidityglass transition temperatureratioprobiotic viabilitylactic acid bacteriastrategyCell Membranecryoprotectantcollapse temperatureFreeze-Drying Resistancefreeze-dryingoctadecenoic acidenzyme activitiescontrol groupcell membrane characteristics
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