Broad-Specificity Immunoassay for Simultaneous Detection of Ochratoxins A, B, and C in Millet and Maize
journal contribution
posted on 2017-05-23, 00:00 authored by Yaqiong Zhang, Lanteng Wang, Xing Shen, Xiaoqun Wei, Xinan Huang, Yingju Liu, Xiulan Sun, Zhanhui Wang, Yuanming Sun, Zhenlin Xu, Sergei A. Eremin, Hongtao LeiOchratoxins
A, B, and C (OTA, OTB, and OTC) can be found in cereals
and feeds; the simultaneous detection of these ochratoxins holds a
great need in food safety. In this study, four antibodies raised from
two ochrotoxin haptens and two coating antigens were compared, and
then a sensitive and broad-specificity enzyme-linked immunosorbent
assay (ELISA) was established for the simultaneous determination of
three ochratoxins, where the detection limits were 0.005, 0.001, and
0.001 ng/mL for OTA, OTB, and OTC, respectively, and recoveries of
three ochratoxins were between 84.3% and 111.7%. This developed method
had been successfully applied to detect ochratoxins in both millet
and maize. Molecular modeling revealed that the broad-specificity
was related with the chlorine electronegativity on OTA and OTC and
the potential of the acetyl ester group on OTC. The proposed ELISA
can be used for simultaneous detection of three ochratoxins.
