bc050250a_si_001.pdf (2.02 MB)

Bright Oligothiophene N-Succinimidyl Esters for Efficient Fluorescent Labeling of Proteins and Oligonucleotides

Download (2.02 MB)
journal contribution
posted on 18.01.2006, 00:00 by Giovanna Barbarella, Massimo Zambianchi, Alfredo Ventola, Eduardo Fabiano, Fabio Della Sala, Giuseppe Gigli, Marco Anni, Andrea Bolognesi, Letizia Polito, Marina Naldi, Massimo Capobianco
The synthesis of multicolor fluorescent oligothiophene N-succinimidyl esters (TSEs) is reported, and their optical properties are discussed with the aid of ab initio calculations. The esters were coupled to proteins and to 3‘-amino-modified oligonucleotides in mild conditions and with similar modalities. A comparative study of the bioconjugate of IgG1 anti-CD3 antibody labeled with a blue fluorescent TSE and with fluorescein isothiocyanate (FITC) is reported, showing that the former achieves higher photoluminescence intensity and optical stability than the latter. Fluorescence resonance energy transfer experiments with TSE-labeled oligonucleotides and examples of cellular imaging via TSE-labeled proteins are reported.

History

Exports