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Boron Binding with the Quorum Sensing Signal AI-2 and Analogues

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journal contribution
posted on 22.07.2004, 00:00 by Martin F. Semmelhack, Shawn R. Campagna, Charlotte Hwa, Michael J. Federle, Bonnie L. Bassler
The unstable bacterial metabolic product, DPD, and the related natural product, laurencione, are shown to have a high affinity for borate complexation, through the hydrated analogue. The boron complex of DPD is Vibrio harveyi AI-2, an interspecies quorum sensing signal in bacteria, and an affinity column with a borate resin is effective in providing the first method for concentrating and purifying V. harveyi AI-2 from the biosynthetic product.