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Biotinylated Glycopolymers Synthesized by Atom Transfer Radical Polymerization

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journal contribution
posted on 14.08.2006, 00:00 by Vimary Vázquez-Dorbatt, Heather D. Maynard
Biotinylated glycopolymers that bind to the protein streptavidin were synthesized by atom transfer radical polymerization (ATRP). Poly(methacrylate)s with pendent N-acetyl-d-glucosamines were prepared by polymerizing the protected monomer, followed by deprotection. Alternatively, the unprotected monomer was directly polymerized. Both paths provided well-defined glycopolymers with narrow molecular weight distributions (PDI = 1.07−1.23). The number-average molecular weights determined by gel permeation chromatography increased with increasing initial monomer-to-initiator ratios. The polymers were synthesized using a biotin-functionalized initiator for ATRP. Confirmation of the end group and binding to the protein streptavidin was achieved by 1H NMR and surface plamon resonance.