posted on 2021-08-09, 15:09authored byLibin Sun, Guang Xin, Zhenshan Hou, Xuemei Zhao, Heran Xu, Xiujing Bao, Rongrong Xia, Yunting Li, Li Li
In this study, headspace solid-phase
microextraction-gas chromatography–mass
spectrometry, multivariate analyses, and transcriptomics were used
to explore the biosynthesis of key volatiles and the formation of
spores in Lentinula (L.) edodes. Among the 50 volatiles identified,
1-octen-3-ol, phenethyl alcohol, and several esters were considered
key aromas because of their higher odor activity values. Eleven volatiles
were screened as biomarkers by orthogonal partial least squares discriminant
analysis, and hierarchical cluster analysis showed that these biomarkers
could represent all volatiles to distinguish the spore release stage.
The activities of lipoxygenase (LOX), hydroperoxide lyase, alcohol
dehydrogenase, and alcohol acyltransferase were higher in L. edodes with spore release. Moreover, linolenic
acid and phenylalanine metabolism were involved in aroma biosynthesis.
One LOX-related gene and five aryl alcohol dehydrogenase-related genes
could regulate the biosynthesis of 1-octen-3-ol, phenethyl alcohol,
and phenylacetaldehyde. In addition, several key genes were involved
in meiosis to regulate sporulation.