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Biosynthesis of Tetronates by a Nonribosomal Peptide Synthetase–Polyketide Synthase System

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posted on 2023-03-06, 14:05 authored by Wenya Tian, Xinru Chen, Jun Zhang, Mengmeng Zheng, Guangzheng Wei, Zixin Deng, Xudong Qu
A cryptic tetronate biosynthetic pathway was identified in Kitasatospora niigatensis DSM 44781 via heterologous expression. Distinct from the currently known biosynthetic pathways, this system utilizes a partially functional nonribosomal peptide synthetase and a broadly selective polyketide synthase to direct the assembly and lactonization of the tetronate scaffold. By employing a permissive crotonyl-CoA reductase/carboxylase to provide different extender units, seven new tetronates (kitaniitetronins A–G) were obtained via precursor-directed biosynthesis.

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